Sunday, December 7, 2014

Tuesday & Thurday, 2nd & 4th December 2014

Tuesday's class went for only one hour while Thursday's class went for 2 hours. And we managed to finish learning the topic on Taxonomy. 

I concluded this week's lesson with a mindmap. 


New things that I've learnt 

1- Phylogenetic -->> study of evolutionary relationships among groups of organisms

2- Phylogeny -->> history of organismal lineages as they change through time.

3- Serology -->> The analysis of the contents and properties of blood serum

4- ELISA (Enzyme-linked immunosorbent assay) -->> a rapid immunochemical test that involves an enzyme used for measuring a wide variety of tests of body fluids. ELISA test detect substances that have antigenic properties, primarily proteins rather than small molecules and ions.

5- Western Blotting -->> An assay that detects specific proteins within a protein mixture by a multistep process.

6- Phage Typing -->> the process of identifying a species of bacterium according to the type of virus that attacks it.

7- Fatty Acid Profiles -->> considered as chemotaxonomic markers to define groups of various taxonomic ranks in flowering plants, trees and other embryophytes.

8- Nucleic Acid Hybridization -->> A technique in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences.

9- Southern Blotting -->> a procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which assay by hybridization may be carried out.

10- DNA Chips (also called "DNA microarray") -->> A small solid support, usually a membrane or glass slide, on which sequences of DNA are fixed in an orderly arrangement. DNA microarrays are used for rapid surveys of the expression of many genes simultaneously, as the sequences contained on a single microarray can number in the thousands. 

11- Ribotyping -->> involves the fingerprinting of genomic DNA restriction fragments that contain all or part of the genes coding for the 16S and 23S rRNA. By digesting the genes with a specific restriction enzyme, fragments of different lengths are generated.

12- FISH (Fluorescent In Situ Hybridisation) -->> a laboratory technique for detecting and locating a specific DNA sequence on a chromosome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it.

13- Dichotomous Keys -->> A reference tool where a series of choices between alternative characters leads progressively to the identification of them species.

14- Cladograms -->>  a diagram depicting patterns of shared characteristics of variou organisms. 


I missed Friday's class because I had to go on a trip for my co-curriculum but from the 

look of it (the assignments that my friends had been assigned to by Dr Wan) it seemed 

like they had fun with the websites that they have to use for the assignments. 


Hmmm, about Taxonomy.. It made me realized that there are actually A LOT of methods 

can be used to classify and name the microorganisms. I thought it was simple as that but 

nope, I was wrong all along. Happy learning, Dayana~ 



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